A three-allele RFLP recognised by an anonymous sequence localised to 3p14-p21 [D3S11 (E41)]

Human gastrin (GAS) RFLP recognised by digestion with BamHI or EcoRI.

SOURCE AUD DESCRIPTION OF CLONE: The human gastrin gene cDNA insert in clone pHG529 (1) is 463 bp in length. This excludes the poly (dC) tail* and includes 8 nucleotides of 5' untranslated region, the entire coding region of 303 nucleotides, a 3 1 untranslated region of 102 nucleotides and a poly (A) track of 50 residues.

An anonymous single copy chromosome 21 probe, DS21D2, associated with a frequent RFLP.

SOURCE/DESCRIPTION An EcoRI 4.0 kb single copy fragment insert into XgtWES Xb isolated from the chromosome. 21 flow-sorted library of Krumlauf et al (1982). POLYMORPHISM TaqI (T-J-CGA) identifies 2 invariant bands and a simple 2 allele polymorphism with a band at 8.4 and/or 5.8 kb. FREQUENCY St-ndled 10 unrelated individuals. 8.4 kb allele (Al) 0.65 5.8 kb allele (A2) 0.35 CHROMOSOMAL LOCALISAT...

Chromosome 3p14 homozygous deletions and sequence analysis of FRA3B.

Loss of heterozygosity (LOH) involving 3p occurs in many carcinomas but is complicated by the identification of four distinct homozygous deletion regions. One putative target, 3p14.2, contains the common fragile site, FRA3B, a hereditary renal carcinoma-associated 3;8 translocation and the candidate tumor suppressor gene, FHIT. Using a approximately 300 kb comsid/lambda contig, we identified ho...

RFLP identified by the anonymous DNA segment FZ IV E11 at 1p23 [HGM9 no.D1S87].

Identification of the CSN1S1 allele in Indian goats by the PCR-RFLP method.

The allelic distributions of the CSN1S1 (αs1-casein) in the Indian goats are quite different from European goat breeds. Majority of Indian goat breeds and non-descript goats carry A, B, E and F alleles at αs1-casein locus, as found by analysing both DNA and protein levels. However, A and B alleles, known to be associated with better casein yield, were observed in the highest proportion in all t...